Structural dynamics of creatine kinase
نویسندگان
چکیده
منابع مشابه
NOVEL REAGENTS FOR CREATINE KINASE
The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
متن کاملStructural changes of creatine kinase upon substrate binding.
Small-angle x-ray scattering was used to investigate structural changes upon binding of individual substrates or a transition state analog complex (TSAC; Mg-ADP, creatine, and KNO3) to creatine kinase (CK) isoenzymes (dimeric muscle-type (M)-CK and octameric mitochondrial (Mi)-CK) and monomeric arginine kinase (AK). Considerable changes in the shape and the size of the molecules occurred upon b...
متن کاملCreatine and creatine kinase measurement.
*Many methods for measuring creatine are known, the principal ones being direct calorimetric assay (l), indirect calorimetric assay (2), and destruction with bacterial adaptive enzymes (3). However, none of these methods are specific nor are they sensitive enough to detect very small amounts of creatine. rIhese assays have been used to examine urine and serum for creatine content, but, because ...
متن کاملAn interlaboratory study of creatine kinase and creatine kinase isoenzymes.
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire regarding methodology. The specimens were prepared from an homogenate of human myocardium that had been centrifuged, dialyzed, and added to a matrix...
متن کاملnovel reagents for creatine kinase
the purpose of this investigation was to develop a simple colorimetric method for creatine kinase (ck). the new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and adp, with different glyoxal compounds. hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were s...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1996
ISSN: 0108-7673
DOI: 10.1107/s0108767396079998